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Dr.
 
Thierry
 
TRON

Portrait de Dr. Thierry Tron
Dr.
Thierry
TRON
AMU iSm2 Service 342
Campus Scientifique de St Jérôme
13397
Marseille cedex 20
Téléphone
Fax
0491 28 84 40
Parcours

EDUCATION

Habilitation to supervise research, Aix Marseille University, 2010

PhD, cellular biology & microbiology, Aix Marseille University, 1991

M. S., microbiology (molecular biology), Aix Marseille University, 1986

B. S., biochemistry, Aix Marseille University, 1984

PROFESSIONAL EXPERIENCE

09/2016-        Head of the BiosCiences laboratory, ISM2, Aix Marseille University, France

2014               CNRS research director (DR2), Aix Marseille University, France

1998-2014     CNRS researcher (CR1) , Aix Marseille University, France

1994-1998     CNRS researcher (CR2) , Aix Marseille University, France

1991-1994     Human Frontier PostDoc research fellow, Dartmouth Medical School, USA

1991               FEBS research fellow (3 months), Biochemistry Dept., Bologne University, Italy

1991               Fondation pour la Recherche Médicale PostDoc (7 months), CNRS Marseille, France

1988-1990     European Union Mobility fellow, Catholic University, Louvain la Neuve, Belgium

Enseignements

PhD level: Doctorate School of Industrial Biotechnology, Federico II University, Napoli : lectures on heterologous expression of metalloenzymes, artificial enzymes, 2012, 2014, 2016.

M. S.level: EU FP7 funded summer schools, ChemBioMeme (2010-2012) and BioMaSolUti (2013-2014) : lectures on heterologous expression of metalloenzymes, artificial enzymes, hybrid photo-catalysts.

Thématiques

My research interests are in the area of biochemistry at the interface between biological, catalytic and inorganic chemistry. With my group we are interested in studying metalloenzymes, in particular copper containing oxidases, that we functionalize with different nano-objects (e. g. transition metal complexes) to obtain hybrid catalysts with original properties. We are currently developing researches on the following topics: 1) bioinspired photo-oxidases allowing a renewable Oxygen Atom Tranfer Reaction, 2) original hybrid Pd-based oxygenases as novel catalysts for environmentally friendly industrial processes, 3) surface-ordered enzyme for high performance bio-cathodes, and 4) surface-modified enzymes with an efficient control of the selectivity.

 

Catalysts based on surface functionalized laccases

with Yasmina Mekmouche, Viviane Robert, Pierre Rousselot Pailley, Jalila Simaan

 

Laccases are well known biocatalysts with great robustness, high oxidation power and substrate versatility.[1] Their unique set of copper centres - a near-surface located mononuclear type 1 and an embedded tri-nuclear cluster (TNC) made of a type 2 and a binuclear type 3 - couple the oxidation of substrates (organic or metal ions) to dioxygen reduction.[2] We shape new catalysts based on oriented functionalizations of a laccase surface with different “plug-ins”.
 From our initial demonstrations on bi-molecular systems - a sensitizer/laccase system coupling the light driven four-electron reduction of dioxygen to water[3] to the photo-oxidation of styrene[4] or a Pd(II)/ laccase system competent for the aerobic oxidation of alcohol in mild conditions[5] -  our latest architectures are based on the oriented grafting of the enzyme surface with prospects on olefin oxidation[6] and laccase functionalized electrode materials[7].

 

Collaborations: Prof. Athanassios Coutsolelos (Univ. Crete), Dr. Alan Legoff (Univ. Grenoble-Alpes), Dr. Victor Guallar (Barcelona Supecomputer Center), Prof. Ally Aukauloo (Univ. Paris Saclay), Dr. WInfried Leibl (CEA Saclay)

References

  1. T. Tron, in Encyclopedia of Metalloproteins, Springer, New York, 1066 (2013)
  2. E. Solomon, U. Sundaram, T. Machonkin, Chem. Rev., 96, 2563 (1996)
  3. T. Lazarides et al., J. Am. Chem. Soc. 135, 3095 (2013)
  4. L. Schneider et al., Chem. Sus. Chem., 8, 3048
(2015)
  5. Y. Mekmouche et al., Chem. Sci., 6,1247 (2015)

  6. V. Robert, et al., Chem. Plus Chem., 82, 607 (2017)
  7. N. Lalaoui et al., ACS Catalysis, 6, 1894 (2016)
Responsabilités administratives

Head of the BiosCiences Laboratory (35 staffs: CNRS researchers,  Professors,  Assistant Professors, Engineers, Technicians)

Publications (52)
Reference Résumé graphique HAL

Site directed confinement of laccases in a porous scaffold towards robustness and selectivity

Fangfang Yang, Rénal Backov, Jean-Luc Blin, Bernadett Fáklya, Thierry Tron, Yasmina Mekmouche, Biotechnology Reports, 2021, 31, e00645. <hal-03262272>

Coniferyl Alcohol Radical Detection by the Dirigent Protein At DIR6 Monitored by EPR

Camille Modolo, Lu Ren, Eric Besson, Viviane Robert, Stéphane Gastaldi, Pierre Rousselot‐pailley, Thierry Tron, ChemBioChem, 2021, 22, 992-995. <hal-03178991>

Tracking Light-Induced Electron Transfer towards O2 in a Hybrid Photoredox-Laccase System

Rajaa Farran, Yasmina Mekmouche, Nhat Tam Vo, Christian Herrero, Annamaria Quaranta, Marie Sircoglou, Frédéric Banse, Pierre Rousselot-Pailley, A. Jalila Simaan, Ally Aukauloo, Thierry Tron, Winfried Leibl, iScience, 2021, 102378. <hal-03190584>

Clicked Bifunctional Dendrimeric and Cyclopeptidic Addressable Redox Scaffolds for the Functionalization of Carbon Nanotubes with Redox Molecules and Enzymes

Solène Gentil, Carlo Pifferi, Pierre Rousselot-Pailley, Thierry Tron, Olivier Renaudet, Alan Le Goff, Langmuir, 2021, 37, 1001–1011. <hal-03172169>

Efficiency of Site‐Specific Clicked Laccase–Carbon Nanotubes Biocathodes towards O 2 Reduction

Solène Gentil, Pierre Rousselot‐pailley, Ferran Sancho, Viviane Robert, Yasmina Mekmouche, Victor Guallar, Thierry Tron, Alan Le Goff, Chemistry - A European Journal, 2020, 26, 4798-4804. <hal-03010787>

Molecular docking studies and in vitro degradation of four aflatoxins (AFB 1 , AFB 2 , AFG 1 , and AFG 2 ) by a recombinant laccase from Saccharomyces cerevisiae

Yingli Liu, Huijia Mao, Chuanqin Hu, Thierry Tron, Junfang Lin, Jing Wang, Baoguo Sun, Journal of Food Science, 2020, 85, 1353-1360. <hal-03121524>

A Reversible Electron Relay to Exclude Sacrificial Electron Donors in the Photocatalytic Oxygen Atom Transfer Reaction with O2 in Water

Nhat Tam Vo, Yasmina Mekmouche, Thierry Tron, Régis Guillot, Frédéric Banse, Zakaria Halime, Marie Sircoglou, Winfried Leibl, Ally Aukauloo, Angewandte Chemie International Edition, 2019. <hal-02301619>

Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label

Sybille Tachon, Eugénie Fournier, Christophe Decroos, Pascal Mansuelle, Emilien Etienne, Marc Maresca, Marlène Martinho, Valérie Belle, Thierry Tron, Ariane Jalila Simaan, Molecular Biotechnology, 2019. <hal-02157289>

Corrigendum: Probing the Surface of a Laccase for Clues towards the Design of Chemo-Enzymatic Catalysts

Viviane Robert, Emanuele Monza, Lionel Tarrago, Ferran Sancho, Anna De falco, Ludovic Schneider, Eloïne Npetgat ngoutane, Yasmina Mekmouche, Pierre Rousselot Pailley, A. Jalila Simaan, Victor Guallar, Thierry Tron, ChemPlusChem, 2018, 83, 831-831. <hal-02929686>

Controlling the polymerization of coniferyl alcohol with cyclodextrins

Lionel Tarrago, Camille Modolo, Mehdi Yemloul, Viviane Robert, Pierre Rousselot-Pailley, Thierry Tron, New Journal of Chemistry, 2018, 42, 11770-11775. <cea-01939414>

Pages

Chapitres d'ouvrages (1)
Reference Résumé graphique HAL

Simeng Zhou, Pierre Rousselot-Pailley, Lu Ren, Yolande Charmasson, Elise Courvoisier Dezord, et al.. Production and manipulation of blue copper oxidases for technological applications. Methods in Enzymology, pp.17-61, 2018, ⟨10.1016/bs.mie.2018.10.015⟩. ⟨hal-02396850⟩