Dr. Thierry TRON
Dr.
Thierry
TRON
AMU iSm2 Service 342
Campus Scientifique de St Jérôme
13397
Marseille cedex 20
Téléphone :
Fax :
0491 28 84 40
Courriel :
Parcours :
EDUCATION
Habilitation to supervise research, Aix Marseille University, 2010
PhD, cellular biology & microbiology, Aix Marseille University, 1991
M. S., microbiology (molecular biology), Aix Marseille University, 1986
B. S., biochemistry, Aix Marseille University, 1984
PROFESSIONAL EXPERIENCE
09/2016- Head of the BiosCiences laboratory, ISM2, Aix Marseille University, France
2014 CNRS research director (DR2), Aix Marseille University, France
1998-2014 CNRS researcher (CR1) , Aix Marseille University, France
1994-1998 CNRS researcher (CR2) , Aix Marseille University, France
1991-1994 Human Frontier PostDoc research fellow, Dartmouth Medical School, USA
1991 FEBS research fellow (3 months), Biochemistry Dept., Bologne University, Italy
1991 Fondation pour la Recherche Médicale PostDoc (7 months), CNRS Marseille, France
1988-1990 European Union Mobility fellow, Catholic University, Louvain la Neuve, Belgium
Enseignements :
PhD level: Doctorate School of Industrial Biotechnology, Federico II University, Napoli : lectures on heterologous expression of metalloenzymes, artificial enzymes, 2012, 2014, 2016.
M. S.level: EU FP7 funded summer schools, ChemBioMeme (2010-2012) and BioMaSolUti (2013-2014) : lectures on heterologous expression of metalloenzymes, artificial enzymes, hybrid photo-catalysts.
Thématiques :
My research interests are in the area of biochemistry at the interface between biological, catalytic and inorganic chemistry. With my group we are interested in studying metalloenzymes, in particular copper containing oxidases, that we functionalize with different nano-objects (e. g. transition metal complexes) to obtain hybrid catalysts with original properties. We are currently developing researches on the following topics: 1) bioinspired photo-oxidases allowing a renewable Oxygen Atom Tranfer Reaction, 2) original hybrid Pd-based oxygenases as novel catalysts for environmentally friendly industrial processes, 3) surface-ordered enzyme for high performance bio-cathodes, and 4) surface-modified enzymes with an efficient control of the selectivity.
Catalysts based on surface functionalized laccases
with Yasmina Mekmouche, Viviane Robert, Pierre Rousselot Pailley, Jalila Simaan
Laccases are well known biocatalysts with great robustness, high oxidation power and substrate versatility.[1] Their unique set of copper centres - a near-surface located mononuclear type 1 and an embedded tri-nuclear cluster (TNC) made of a type 2 and a binuclear type 3 - couple the oxidation of substrates (organic or metal ions) to dioxygen reduction.[2] We shape new catalysts based on oriented functionalizations of a laccase surface with different “plug-ins”.
From our initial demonstrations on bi-molecular systems - a sensitizer/laccase system coupling the light driven four-electron reduction of dioxygen to water[3] to the photo-oxidation of styrene[4] or a Pd(II)/ laccase system competent for the aerobic oxidation of alcohol in mild conditions[5] - our latest architectures are based on the oriented grafting of the enzyme surface with prospects on olefin oxidation[6] and laccase functionalized electrode materials[7].
Collaborations: Prof. Athanassios Coutsolelos (Univ. Crete), Dr. Alan Legoff (Univ. Grenoble-Alpes), Dr. Victor Guallar (Barcelona Supecomputer Center), Prof. Ally Aukauloo (Univ. Paris Saclay), Dr. WInfried Leibl (CEA Saclay)
References
- T. Tron, in Encyclopedia of Metalloproteins, Springer, New York, 1066 (2013)
- E. Solomon, U. Sundaram, T. Machonkin, Chem. Rev., 96, 2563 (1996)
- T. Lazarides et al., J. Am. Chem. Soc. 135, 3095 (2013)
- L. Schneider et al., Chem. Sus. Chem., 8, 3048
(2015)
- Y. Mekmouche et al., Chem. Sci., 6,1247 (2015)
- V. Robert, et al., Chem. Plus Chem., 82, 607 (2017)
- N. Lalaoui et al., ACS Catalysis, 6, 1894 (2016)
Responsabilités administratives :
Head of the BiosCiences Laboratory (35 staffs: CNRS researchers, Professors, Assistant Professors, Engineers, Technicians)
Publications (52)
Reference
Résumé graphique
HAL
Controlled Immobilization of a Palladium Complex/Laccase Hybrid into a Macrocellular Siliceous Host
Fangfang Yang, Pierre Rousselot‐pailley, Cendrine Nicoletti, A. Jalila Simaan, Bruno Faure, Elise Courvoisier‐dezord, Agnès Amouric, Yolande Charmasson, Rénal R Backov, Thierry Tron, Yasmina Mekmouche, ChemPlusChem, 2023, 88, e202300156. <hal-04109580>
✓
Laccase-catalyzed functionalization of phenol-modified carbon nanotubes: from grafting of metallopolyphenols to enzyme self-immobilization.
Umberto Contaldo, Solène Gentil, Elise Courvoisier‐dezord, Pierre Rousselot Pailley, Fabrice Thomas, Thierry Tron, Alan Le Goff, Journal of Materials Chemistry A, 2023, 11, 10850-10856. <hal-04119457>
✓
Investigation of dirigent like domains from bacterial genomes
Merlin Bardin, Pierre Rousselot-Pailley, Thierry Tron, Viviane Robert, BMC Bioinformatics, 2022, 23, 313. <hal-03957114>
✓
Photocatalytic generation of a non-heme Fe(III)-hydroperoxo species with O$_2$ in water for oxygen atom transfer reaction
Eva Pugliese, Nhat Tam Vo, Alain Boussac, Frédéric Banse, Yasmina Mekmouche, A. Jalila Simaan, Thierry Tron, Philipp Gotico, Marie Sircoglou, Zakaria Halime, Winfried Leibl, Ally Aukauloo, Chemical Science, 2022, 2012. <hal-03795563>
✓
2D and 3D Maximum-Quantum NMR and diffusion spectroscopy for the characterization of enzymatic reaction mixtures
Elena Piersanti, Claudio Righetti, David Ribeaucourt, A. Jalila Simaan, Yasmina Mekmouche, Mickael Lafond, Jean-Guy Berrin, Thierry Tron, Mehdi Yemloul, Analyst, 2022, 147, 2515-2522. <hal-03652618>
✓
Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles
Lu Ren, Hongtao Ji, Karine Heuzé, Bruno Faure, Emilie Genin, Pierre Rousselot Pailley, Thierry Tron, Colloids and Surfaces B: Biointerfaces, 2021, 206, 111963. <hal-03405996>
✓
Site directed confinement of laccases in a porous scaffold towards robustness and selectivity
Fangfang Yang, Rénal Backov, Jean-Luc Blin, Bernadett Fáklya, Thierry Tron, Yasmina Mekmouche, Biotechnology Reports, 2021, 31, e00645. <hal-03262272>
✓
Coniferyl Alcohol Radical Detection by the Dirigent Protein At DIR6 Monitored by EPR
Camille Modolo, Lu Ren, Eric Besson, Viviane Robert, Stéphane Gastaldi, Pierre Rousselot‐pailley, Thierry Tron, ChemBioChem, 2021, 22, 992-995. <hal-03178991>
✓
Tracking Light-Induced Electron Transfer towards O2 in a Hybrid Photoredox-Laccase System
Rajaa Farran, Yasmina Mekmouche, Nhat Tam Vo, Christian Herrero, Annamaria Quaranta, Marie Sircoglou, Frédéric Banse, Pierre Rousselot-Pailley, A. Jalila Simaan, Ally Aukauloo, Thierry Tron, Winfried Leibl, iScience, 2021, 102378. <hal-03190584>
✓
Clicked Bifunctional Dendrimeric and Cyclopeptidic Addressable Redox Scaffolds for the Functionalization of Carbon Nanotubes with Redox Molecules and Enzymes
Solène Gentil, Carlo Pifferi, Pierre Rousselot-Pailley, Thierry Tron, Olivier Renaudet, Alan Le Goff, Langmuir, 2021, 37, 1001-1011. <hal-03132807>
✓
Pages
Communications orales et par affiche (1)
Reference
Résumé graphique
HAL
Lise Molinelli, Maya Belghazi, Thierry Tron, Lionel Tarrago. A chemoproteomic approach to identify redox-active methionines in secreted proteins of
saprophytic fungi during biomass degradation. Club oxydase 2021, 2021, Grenoble, France. ⟨hal-03522965⟩
✓
Chapitres d'ouvrages (1)
Reference
Résumé graphique
HAL
Simeng Zhou, Pierre Rousselot-Pailley, Lu Ren, Yolande Charmasson, Elise Courvoisier Dezord, et al.. Production and manipulation of blue copper oxidases for technological applications. Methods in Enzymology, pp.17-61, 2018, ⟨10.1016/bs.mie.2018.10.015⟩. ⟨hal-02396850⟩
✖
EDUCATION
Habilitation to supervise research, Aix Marseille University, 2010
PhD, cellular biology & microbiology, Aix Marseille University, 1991
M. S., microbiology (molecular biology), Aix Marseille University, 1986
B. S., biochemistry, Aix Marseille University, 1984
PROFESSIONAL EXPERIENCE
09/2016- Head of the BiosCiences laboratory, ISM2, Aix Marseille University, France
2014 CNRS research director (DR2), Aix Marseille University, France
1998-2014 CNRS researcher (CR1) , Aix Marseille University, France
1994-1998 CNRS researcher (CR2) , Aix Marseille University, France
1991-1994 Human Frontier PostDoc research fellow, Dartmouth Medical School, USA
1991 FEBS research fellow (3 months), Biochemistry Dept., Bologne University, Italy
1991 Fondation pour la Recherche Médicale PostDoc (7 months), CNRS Marseille, France
1988-1990 European Union Mobility fellow, Catholic University, Louvain la Neuve, Belgium
PhD level: Doctorate School of Industrial Biotechnology, Federico II University, Napoli : lectures on heterologous expression of metalloenzymes, artificial enzymes, 2012, 2014, 2016.
M. S.level: EU FP7 funded summer schools, ChemBioMeme (2010-2012) and BioMaSolUti (2013-2014) : lectures on heterologous expression of metalloenzymes, artificial enzymes, hybrid photo-catalysts.
My research interests are in the area of biochemistry at the interface between biological, catalytic and inorganic chemistry. With my group we are interested in studying metalloenzymes, in particular copper containing oxidases, that we functionalize with different nano-objects (e. g. transition metal complexes) to obtain hybrid catalysts with original properties. We are currently developing researches on the following topics: 1) bioinspired photo-oxidases allowing a renewable Oxygen Atom Tranfer Reaction, 2) original hybrid Pd-based oxygenases as novel catalysts for environmentally friendly industrial processes, 3) surface-ordered enzyme for high performance bio-cathodes, and 4) surface-modified enzymes with an efficient control of the selectivity.
Catalysts based on surface functionalized laccases
with Yasmina Mekmouche, Viviane Robert, Pierre Rousselot Pailley, Jalila Simaan
Laccases are well known biocatalysts with great robustness, high oxidation power and substrate versatility.[1] Their unique set of copper centres - a near-surface located mononuclear type 1 and an embedded tri-nuclear cluster (TNC) made of a type 2 and a binuclear type 3 - couple the oxidation of substrates (organic or metal ions) to dioxygen reduction.[2] We shape new catalysts based on oriented functionalizations of a laccase surface with different “plug-ins”. From our initial demonstrations on bi-molecular systems - a sensitizer/laccase system coupling the light driven four-electron reduction of dioxygen to water[3] to the photo-oxidation of styrene[4] or a Pd(II)/ laccase system competent for the aerobic oxidation of alcohol in mild conditions[5] - our latest architectures are based on the oriented grafting of the enzyme surface with prospects on olefin oxidation[6] and laccase functionalized electrode materials[7].
Collaborations: Prof. Athanassios Coutsolelos (Univ. Crete), Dr. Alan Legoff (Univ. Grenoble-Alpes), Dr. Victor Guallar (Barcelona Supecomputer Center), Prof. Ally Aukauloo (Univ. Paris Saclay), Dr. WInfried Leibl (CEA Saclay)
References
- T. Tron, in Encyclopedia of Metalloproteins, Springer, New York, 1066 (2013)
- E. Solomon, U. Sundaram, T. Machonkin, Chem. Rev., 96, 2563 (1996)
- T. Lazarides et al., J. Am. Chem. Soc. 135, 3095 (2013)
- L. Schneider et al., Chem. Sus. Chem., 8, 3048 (2015)
- Y. Mekmouche et al., Chem. Sci., 6,1247 (2015)
- V. Robert, et al., Chem. Plus Chem., 82, 607 (2017)
- N. Lalaoui et al., ACS Catalysis, 6, 1894 (2016)
Head of the BiosCiences Laboratory (35 staffs: CNRS researchers, Professors, Assistant Professors, Engineers, Technicians)
| Reference | Résumé graphique | HAL |
|---|---|---|
|
Controlled Immobilization of a Palladium Complex/Laccase Hybrid into a Macrocellular Siliceous Host |
|
✓ |
|
Laccase-catalyzed functionalization of phenol-modified carbon nanotubes: from grafting of metallopolyphenols to enzyme self-immobilization. |
|
✓ |
|
Investigation of dirigent like domains from bacterial genomes |
|
✓ |
|
Photocatalytic generation of a non-heme Fe(III)-hydroperoxo species with O$_2$ in water for oxygen atom transfer reaction |
|
✓ |
|
2D and 3D Maximum-Quantum NMR and diffusion spectroscopy for the characterization of enzymatic reaction mixtures |
|
✓ |
|
Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles |
|
✓ |
|
Site directed confinement of laccases in a porous scaffold towards robustness and selectivity |
|
✓ |
|
Coniferyl Alcohol Radical Detection by the Dirigent Protein At DIR6 Monitored by EPR |
|
✓ |
|
Tracking Light-Induced Electron Transfer towards O2 in a Hybrid Photoredox-Laccase System |
|
✓ |
|
Clicked Bifunctional Dendrimeric and Cyclopeptidic Addressable Redox Scaffolds for the Functionalization of Carbon Nanotubes with Redox Molecules and Enzymes |
|
✓ |
Pages
| Reference | Résumé graphique | HAL |
|---|---|---|
|
Lise Molinelli, Maya Belghazi, Thierry Tron, Lionel Tarrago. A chemoproteomic approach to identify redox-active methionines in secreted proteins of |
|
✓ |
| Reference | Résumé graphique | HAL |
|---|---|---|
|
Simeng Zhou, Pierre Rousselot-Pailley, Lu Ren, Yolande Charmasson, Elise Courvoisier Dezord, et al.. Production and manipulation of blue copper oxidases for technological applications. Methods in Enzymology, pp.17-61, 2018, ⟨10.1016/bs.mie.2018.10.015⟩. ⟨hal-02396850⟩ |
|
✖ |