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Portrait de Dr. Thierry Tron
AMU iSm2 Service 342
Campus Scientifique de St Jérôme
Marseille cedex 20
0491 28 84 40


Habilitation to supervise research, Aix Marseille University, 2010

PhD, cellular biology & microbiology, Aix Marseille University, 1991

M. S., microbiology (molecular biology), Aix Marseille University, 1986

B. S., biochemistry, Aix Marseille University, 1984


09/2016-        Head of the BiosCiences laboratory, ISM2, Aix Marseille University, France

2014               CNRS research director (DR2), Aix Marseille University, France

1998-2014     CNRS researcher (CR1) , Aix Marseille University, France

1994-1998     CNRS researcher (CR2) , Aix Marseille University, France

1991-1994     Human Frontier PostDoc research fellow, Dartmouth Medical School, USA

1991               FEBS research fellow (3 months), Biochemistry Dept., Bologne University, Italy

1991               Fondation pour la Recherche Médicale PostDoc (7 months), CNRS Marseille, France

1988-1990     European Union Mobility fellow, Catholic University, Louvain la Neuve, Belgium


PhD level: Doctorate School of Industrial Biotechnology, Federico II University, Napoli : lectures on heterologous expression of metalloenzymes, artificial enzymes, 2012, 2014, 2016.

M. S.level: EU FP7 funded summer schools, ChemBioMeme (2010-2012) and BioMaSolUti (2013-2014) : lectures on heterologous expression of metalloenzymes, artificial enzymes, hybrid photo-catalysts.


My research interests are in the area of biochemistry at the interface between biological, catalytic and inorganic chemistry. With my group we are interested in studying metalloenzymes, in particular copper containing oxidases, that we functionalize with different nano-objects (e. g. transition metal complexes) to obtain hybrid catalysts with original properties. We are currently developing researches on the following topics: 1) bioinspired photo-oxidases allowing a renewable Oxygen Atom Tranfer Reaction, 2) original hybrid Pd-based oxygenases as novel catalysts for environmentally friendly industrial processes, 3) surface-ordered enzyme for high performance bio-cathodes, and 4) surface-modified enzymes with an efficient control of the selectivity.


Catalysts based on surface functionalized laccases

with Yasmina Mekmouche, Viviane Robert, Pierre Rousselot Pailley, Jalila Simaan


Laccases are well known biocatalysts with great robustness, high oxidation power and substrate versatility.[1] Their unique set of copper centres - a near-surface located mononuclear type 1 and an embedded tri-nuclear cluster (TNC) made of a type 2 and a binuclear type 3 - couple the oxidation of substrates (organic or metal ions) to dioxygen reduction.[2] We shape new catalysts based on oriented functionalizations of a laccase surface with different “plug-ins”.
 From our initial demonstrations on bi-molecular systems - a sensitizer/laccase system coupling the light driven four-electron reduction of dioxygen to water[3] to the photo-oxidation of styrene[4] or a Pd(II)/ laccase system competent for the aerobic oxidation of alcohol in mild conditions[5] -  our latest architectures are based on the oriented grafting of the enzyme surface with prospects on olefin oxidation[6] and laccase functionalized electrode materials[7].


Collaborations: Prof. Athanassios Coutsolelos (Univ. Crete), Dr. Alan Legoff (Univ. Grenoble-Alpes), Dr. Victor Guallar (Barcelona Supecomputer Center), Prof. Ally Aukauloo (Univ. Paris Saclay), Dr. WInfried Leibl (CEA Saclay)


  1. T. Tron, in Encyclopedia of Metalloproteins, Springer, New York, 1066 (2013)
  2. E. Solomon, U. Sundaram, T. Machonkin, Chem. Rev., 96, 2563 (1996)
  3. T. Lazarides et al., J. Am. Chem. Soc. 135, 3095 (2013)
  4. L. Schneider et al., Chem. Sus. Chem., 8, 3048
  5. Y. Mekmouche et al., Chem. Sci., 6,1247 (2015)

  6. V. Robert, et al., Chem. Plus Chem., 82, 607 (2017)
  7. N. Lalaoui et al., ACS Catalysis, 6, 1894 (2016)
Responsabilités administratives

Head of the BiosCiences Laboratory (35 staffs: CNRS researchers,  Professors,  Assistant Professors, Engineers, Technicians)

Publications (52)
Reference Résumé graphique HAL

Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay

Lise Molinelli, Elodie Drula, Jean-Charles Gaillard, David Navarro, Jean Armengaud, Jean-Guy Berrin, Thierry Tron, Lionel Tarrago, Applied and Environmental Microbiology, 2024. <hal-04468884>

Tuning Chemoenzymatic Pd/Laccase Conformation Toward Optimized Heterogeneous Aerobic Oxidation

Fangfang Yang, Pierre Rousselot Pailley, Rénal Backov, Elise Courvoisier-Dezord, Agnès Amouric, Thierry Tron, Yasmina Mekmouche, ChemBioChem, 2024, 25. <hal-04460072>

Controlled Immobilization of a Palladium Complex/Laccase Hybrid into a Macrocellular Siliceous Host

Fangfang Yang, Pierre Rousselot-Pailley, Cendrine Nicoletti, A. Jalila Simaan, Bruno Faure, Elise Courvoisier-Dezord, Agnès Amouric, Yolande Charmasson, Rénal Backov, Thierry Tron, Yasmina Mekmouche, ChemPlusChem, 2023, 88, e202300156. <hal-04105029>

Laccase-catalyzed functionalization of phenol-modified carbon nanotubes: from grafting of metallopolyphenols to enzyme self-immobilization.

Umberto Contaldo, Solène Gentil, Elise Courvoisier‐dezord, Pierre Rousselot Pailley, Fabrice Thomas, Thierry Tron, Alan Le Goff, Journal of Materials Chemistry A, 2023, 11, 10850-10856. <hal-04119457>

Investigation of dirigent like domains from bacterial genomes

Merlin Bardin, Pierre Rousselot-Pailley, Thierry Tron, Viviane Robert, BMC Bioinformatics, 2022, 23, 313. <hal-03957114>

Photocatalytic generation of a non-heme Fe(III)-hydroperoxo species with O$_2$ in water for oxygen atom transfer reaction

Eva Pugliese, Nhat Tam Vo, Alain Boussac, Frédéric Banse, Yasmina Mekmouche, A. Jalila Simaan, Thierry Tron, Philipp Gotico, Marie Sircoglou, Zakaria Halime, Winfried Leibl, Ally Aukauloo, Chemical Science, 2022, 2012. <hal-03795563>

2D and 3D Maximum-Quantum NMR and diffusion spectroscopy for the characterization of enzymatic reaction mixtures

Elena Piersanti, Claudio Righetti, David Ribeaucourt, A. Jalila Simaan, Yasmina Mekmouche, Mickael Lafond, Jean-Guy Berrin, Thierry Tron, Mehdi Yemloul, Analyst, 2022, 147, 2515-2522. <hal-03652618>

Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles

Lu Ren, Hongtao Ji, Karine Heuzé, Bruno Faure, Emilie Genin, Pierre Rousselot Pailley, Thierry Tron, Colloids and Surfaces B: Biointerfaces, 2021, 206, 111963. <hal-03405996>

Site directed confinement of laccases in a porous scaffold towards robustness and selectivity

Fangfang Yang, Rénal Backov, Jean-Luc Blin, Bernadett Fáklya, Thierry Tron, Yasmina Mekmouche, Biotechnology Reports, 2021, 31, e00645. <hal-03262272>

Tracking Light-Induced Electron Transfer towards O2 in a Hybrid Photoredox-Laccase System

Rajaa Farran, Yasmina Mekmouche, Nhat Tam Vo, Christian Herrero, Annamaria Quaranta, Marie Sircoglou, Frédéric Banse, Pierre Rousselot-Pailley, A. Jalila Simaan, Ally Aukauloo, Thierry Tron, Winfried Leibl, iScience, 2021, 102378. <hal-03190584>


Communications orales et par affiche (1)
Reference Résumé graphique HAL

Lise Molinelli, Maya Belghazi, Thierry Tron, Lionel Tarrago. A chemoproteomic approach to identify redox-active methionines in secreted proteins of
saprophytic fungi during biomass degradation. Club oxydase 2021, 2021, Grenoble, France. ⟨hal-03522965⟩

Chapitres d'ouvrages (1)
Reference Résumé graphique HAL

Simeng Zhou, Pierre Rousselot-Pailley, Lu Ren, Yolande Charmasson, Elise Courvoisier Dezord, et al.. Production and manipulation of blue copper oxidases for technological applications. Methods in Enzymology, pp.17-61, 2018, ⟨10.1016/bs.mie.2018.10.015⟩. ⟨hal-02396850⟩