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Mme
 
Clarisse
 
ROBLIN

Portrait de Mme Clarisse Roblin
Mme
Clarisse
ROBLIN
AMU iSm2 Service 342
Campus Scientifique de St Jérôme
13397
Marseille cedex 20
Téléphone

 

 

Entrée
01/01/2017
Soutenance
14/09/2020
Directeur de thèse
Josette PERRIER & Michael LAFOND
Sujet de thèse
Les Ruminococcines C, une nouvelle famille de sactipeptides comme alternative aux antibiotiques conventionnels
Parcours

Publications

Roblin C., Chiumento S., Jacqueline C., Pinloche E., Nicoletti C., Olleik H., Courvoisier-Dezord E., Amouric A., Basset C., Dru L., Ollivier M., Bogey-Lambert A., Vidal N., Atta M., Maresca M., Devillard E., Duarte V., Perrier J., Lafond M. 2021. The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health. Int. J. Mol. Sci., 22, 3253.

https://doi.org/10.3390/ijms22063253

Roblin C.*, Chiumento S.*, Bornet O., Nouailler M., Müller C., Jeannot K., Basset C., Kieffer-Jaquinod S., Couté Y., Torelli S., Le Pape L., Schünemann V., De La Villeon B., Sockeel P., Di Pasquale E., Nicoletti C., Iranzo O., Giardina T., Fons M., Devillard E., Maresca M., Perrier J., Atta M., Guerlesquin F., Lafond M., Duarte V., 2020. The unusual structure of Ruminococcin C1 antimicrobial peptide promotes its potential therapeutic use. P.NA.S., 117 (32) 19168-19177.

https://doi.org/10.1073/pnas.2004045117

Chiumento S.*, Roblin C.*, Kieffer-Jaquinod S.*, Tachon S., Leprètre C., Basset C., Aditiyarini D., Olleik H., Nicoletti C., Bornet O., Iranzo O., Maresca M., Hardré R., Fons M., Giardina T., Devillard E., Guerlesquin F., Couté Y., Atta M., Perrier J., Lafond M., Duarte V., 2019. Ruminococcin C, a promising antibiotic produced by a human gut symbiont. Science Advances 5, eaaw9969.

https://doi.org/10.1126/sciadv.aaw9969

* These authors contributed equally to the publications.

 

Research Experience

 

Institut des Sciences Moléculaires de Marseille,  France (Jan 2017 – June 2020)
Subject: Purification and characterization of the Ruminococcins C, a new family of sactipeptides and an alternative to conventionnal antibiotics
Skills: Peptides purification by chromatography (HPLC and FPLC systems), microbiological practices including on pathogens and anaerobes, identification of antimicrobial mode of action, fluorescence microscopy and image analysis

Advisors: Mickael Lafond & Josette Perrier, Estelle Devillard (Adisseo France SAS)

 

University of California San Diego, USA (Sept. 2015 – April 2016)
Subject: Library screening for antibiotic discovery
Skills: MIC determination, Bacterial Cytological Profiling (BCP) by fluorescence microscopy and image analysis
Advisors: Rob Knight and Joseph Pogliano

 

University of California San Diego, USA (Jan. – June 2015)
Subject: Development of an inducible protein degradation system in E. coli to identify novel targets for antibiotics
Skills: DNA purification, PCR, transfection and cloning, fluorescence microscopy and image analysis
Advisor: Joseph Pogliano

 

University of California San Diego, USA (April – Aug. 2014)
Subject: Characterization of newly isolated caves microorganisms and antibiotics extraction
Skills: 16S rRNA sequencing, chemical extraction and purification by chromatography, fluorescence microscopy
Advisor: Joseph Pogliano

 

McGill University, Montreal, Canada (May – Aug. 2013)
Subject: Implication of p120 RasGAP in netrin-1 mediated axon guidance
Skills: Cell culturing techniques, DNA purification, transfection, protein purification and Western-blot
Advisor: Nathalie Lamarche-Vane
Award: Merit-based CIHR (Canadian Institute of Health Research) scholarship

 

Laboratory of Microbiology Signals and Microenvironment (LMSM), Evreux (May – July. 2012)
Subject: Implication of TspO protein in the tolerance to several environmental stresses of P. fluorescens
Skills: DNA and RNA purification, PCR, RT-PCR
Advisor: Emeline Bouffartigues

 

Education

 

Aix-Marseille Université (2017 – 2020)
Ph.D. in Microbiology (Thesis defended on September 14th 2020)

 

Université Paris Diderot - Paris 7 (2013 – 2015)
M.Sc. in Genetics (with Honors)
Coursework: Genetics, Epigenetic, Genomics, Development, Cellular imaging

 

Université Paris Diderot - Paris 7 (2012 – 2013)
B.Sc. in Natural Sciences (with Honors)
Coursework: Genetics, Molecular biology, Cellular biology, Evolutionary genetics

 

University Institute of Technology, Evreux (2011 – 2012)
University Degree in Technology in Biological and Biochemical Analysis
Coursework: Molecular biology, Proteomics, Microbiology, Immunology, Toxicology, Pharmacology

 

Compiegne Technological University (2009 – 2011)
Engineering school
Coursework: Mathematics, Physics, Chemistry, Thermodynamics, Biology

 

Lycée Jeanne d’Arc, Rouen (2009)
High school diploma (with High honors)
Major in Physics and Chemistry

Distinctions

Best Poster Contribution - 6Th International Symposium AntiMicrobial Peptides - 2018

Most Creative Idea - Young Researchers Contest - Pepper School - 2018

Merit-based scolarship - Canadian Institute of Health Research - 2013

Actions de vulgarisation

Apprentis chercheurs

Ma thèse en 180 secondes : https://www.youtube.com/watch?v=8U1rWTyxX2M

Publications (3)
Reference Résumé graphique HAL

The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health

Clarisse Roblin, Steve Chiumento, Cédric Jacqueline, Eric Pinloche, Cendrine Nicoletti, Hamza Olleik, Elise Courvoisier-Dezord, Agnès Amouric, Christian Basset, Louis Dru, Marie Ollivier, Aurélie Bogey-Lambert, Nicolas Vidal, Mohamed Atta, Marc Maresca, Estelle Devillard, Victor Duarte, Josette Perrier, Mickael Lafond, International Journal of Molecular Sciences, 2021, 22, 3253. <hal-03232443>

The unusual structure of Ruminococcin C1 antimicrobial peptide confers clinical properties

Clarisse Roblin, Steve Chiumento, Olivier Bornet, Matthieu Nouailler, Christina S. Müller, Katy Jeannot, Christian Basset, Sylvie Kieffer-Jaquinod, Yohann Couté, Stéphane Torelli, Laurent Le pape, Volker Schünemann, Hamza Olleik, Bruno de la Villeon, Philippe Sockeel, Eric Di Pasquale, Cendrine Nicoletti, Nicolas Vidal, Leonora Poljak, Olga Iranzo, Thierry Giardina, Michel Fons, Estelle Devillard, Patrice Polard, Marc Maresca, Josette Perrier, Mohamed Atta, Francoise Guerlesquin, Mickaël Lafond, Victor Duarte, Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, 19168-19177. <hal-02920462>

Ruminococcin C, a promising antibiotic produced by a human gut symbiont

Steve Chiumento, Clarisse Roblin, Sylvie Kieffer-Jaquinod, Sybille Tachon, Chloé Leprêtre, Christian Basset, Dwi Aditiyarini, Hamza Olleik, Cendrine Nicoletti, Olivier Bornet, Olga Iranzo, Marc Maresca, Renaud Hardré, Michel Fons, Thierry Giardina, Estelle Devillard, Françoise Guerlesquin, Yohann Couté, Mohamed Atta, Josette Perrier, Mickael Lafond, Victor Duarte, Science Advances, 2019, 5, eaaw9969. <hal-02371747>