Mme Clarisse ROBLIN
Mme
Clarisse
ROBLIN
AMU iSm2 Service 342
Campus Scientifique de St Jérôme
13397
Marseille cedex 20
Phone number :
Date of entry :
01/01/2017
PhD submission :
14/09/2020
PhD supervisor :
Josette PERRIER & Michael LAFOND
PhD subject :
Les Ruminococcines C, une nouvelle famille de sactipeptides comme alternative aux antibiotiques conventionnels
Career path :
Publications
Roblin C., Chiumento S., Jacqueline C., Pinloche E., Nicoletti C., Olleik H., Courvoisier-Dezord E., Amouric A., Basset C., Dru L., Ollivier M., Bogey-Lambert A., Vidal N., Atta M., Maresca M., Devillard E., Duarte V., Perrier J., Lafond M. 2021. The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health. Int. J. Mol. Sci., 22, 3253.
https://doi.org/10.3390/ijms22063253
Roblin C.*, Chiumento S.*, Bornet O., Nouailler M., Müller C., Jeannot K., Basset C., Kieffer-Jaquinod S., Couté Y., Torelli S., Le Pape L., Schünemann V., De La Villeon B., Sockeel P., Di Pasquale E., Nicoletti C., Iranzo O., Giardina T., Fons M., Devillard E., Maresca M., Perrier J., Atta M., Guerlesquin F., Lafond M., Duarte V., 2020. The unusual structure of Ruminococcin C1 antimicrobial peptide promotes its potential therapeutic use. P.NA.S., 117 (32) 19168-19177.
https://doi.org/10.1073/pnas.2004045117
Chiumento S.*, Roblin C.*, Kieffer-Jaquinod S.*, Tachon S., Leprètre C., Basset C., Aditiyarini D., Olleik H., Nicoletti C., Bornet O., Iranzo O., Maresca M., Hardré R., Fons M., Giardina T., Devillard E., Guerlesquin F., Couté Y., Atta M., Perrier J., Lafond M., Duarte V., 2019. Ruminococcin C, a promising antibiotic produced by a human gut symbiont. Science Advances 5, eaaw9969.
https://doi.org/10.1126/sciadv.aaw9969
* These authors contributed equally to the publications.
Research Experience
Institut des Sciences Moléculaires de Marseille, France (Jan 2017 – June 2020)
Subject: Purification and characterization of the Ruminococcins C, a new family of sactipeptides and an alternative to conventionnal antibiotics
Skills: Peptides purification by chromatography (HPLC and FPLC systems), microbiological practices including on pathogens and anaerobes, identification of antimicrobial mode of action, fluorescence microscopy and image analysis
Advisors: Mickael Lafond & Josette Perrier, Estelle Devillard (Adisseo France SAS)
University of California San Diego, USA (Sept. 2015 – April 2016)
Subject: Library screening for antibiotic discovery
Skills: MIC determination, Bacterial Cytological Profiling (BCP) by fluorescence microscopy and image analysis
Advisors: Rob Knight and Joseph Pogliano
University of California San Diego, USA (Jan. – June 2015)
Subject: Development of an inducible protein degradation system in E. coli to identify novel targets for antibiotics
Skills: DNA purification, PCR, transfection and cloning, fluorescence microscopy and image analysis
Advisor: Joseph Pogliano
University of California San Diego, USA (April – Aug. 2014)
Subject: Characterization of newly isolated caves microorganisms and antibiotics extraction
Skills: 16S rRNA sequencing, chemical extraction and purification by chromatography, fluorescence microscopy
Advisor: Joseph Pogliano
McGill University, Montreal, Canada (May – Aug. 2013)
Subject: Implication of p120 RasGAP in netrin-1 mediated axon guidance
Skills: Cell culturing techniques, DNA purification, transfection, protein purification and Western-blot
Advisor: Nathalie Lamarche-Vane
Award: Merit-based CIHR (Canadian Institute of Health Research) scholarship
Laboratory of Microbiology Signals and Microenvironment (LMSM), Evreux (May – July. 2012)
Subject: Implication of TspO protein in the tolerance to several environmental stresses of P. fluorescens
Skills: DNA and RNA purification, PCR, RT-PCR
Advisor: Emeline Bouffartigues
Education
Aix-Marseille Université (2017 – 2020)
Ph.D. in Microbiology (Thesis defended on September 14th 2020)
Université Paris Diderot - Paris 7 (2013 – 2015)
M.Sc. in Genetics (with Honors)
Coursework: Genetics, Epigenetic, Genomics, Development, Cellular imaging
Université Paris Diderot - Paris 7 (2012 – 2013)
B.Sc. in Natural Sciences (with Honors)
Coursework: Genetics, Molecular biology, Cellular biology, Evolutionary genetics
University Institute of Technology, Evreux (2011 – 2012)
University Degree in Technology in Biological and Biochemical Analysis
Coursework: Molecular biology, Proteomics, Microbiology, Immunology, Toxicology, Pharmacology
Compiegne Technological University (2009 – 2011)
Engineering school
Coursework: Mathematics, Physics, Chemistry, Thermodynamics, Biology
Lycée Jeanne d’Arc, Rouen (2009)
High school diploma (with High honors)
Major in Physics and Chemistry
Honour :
PhD Thesis Prize - French Society of Microbiology (SFM) - 2021
Best Poster Contribution - 6Th International Symposium AntiMicrobial Peptides - 2018
Most Creative Idea - Young Researchers Contest - Pepper School - 2018
Merit-based scolarship - Canadian Institute of Health Research - 2013
Scientific outreach efforts :
Apprentis chercheurs
Ma thèse en 180 secondes : https://www.youtube.com/watch?v=8U1rWTyxX2M
Publications (4)
Reference
Graphical abstract
HAL
Evaluation of the Efficiency of Random and Diblock Methacrylate-Based Amphiphilic Cationic Polymers against Major Bacterial Pathogens Associated with Cystic Fibrosis
Magali Casanova, Hamza Olleik, Slim Hdiouech, Clarisse Roblin, Jean-François Cavalier, Vanessa Point, Katy Jeannot, Baptiste Caron, Josette Perrier, Siméon Charriau, Mickael Lafond, Yohann Guillaneuf, Stéphane Canaan, Catherine Lefay, Marc Maresca, Antibiotics, 2023, 12, 120. <hal-03998029>
✓
The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health
Clarisse Roblin, Steve Chiumento, Cédric Jacqueline, Eric Pinloche, Cendrine Nicoletti, Hamza Olleik, Elise Courvoisier-Dezord, Agnès Amouric, Christian Basset, Louis Dru, Marie Ollivier, Aurélie Bogey-Lambert, Nicolas Vidal, Mohamed Atta, Marc Maresca, Estelle Devillard, Victor Duarte, Josette Perrier, Mickael Lafond, International Journal of Molecular Sciences, 2021, 22, 3253. <hal-03232443>
✓
The unusual structure of Ruminococcin C1 antimicrobial peptide confers clinical properties
Clarisse Roblin, Steve Chiumento, Olivier Bornet, Matthieu Nouailler, Christina S. Müller, Katy Jeannot, Christian Basset, Sylvie Kieffer-Jaquinod, Yohann Couté, Stéphane Torelli, Laurent Le pape, Volker Schünemann, Hamza Olleik, Bruno de La Villeon, Philippe Sockeel, Eric Di Pasquale, Cendrine Nicoletti, Nicolas Vidal, Leonora Poljak, Olga Iranzo, Thierry Giardina, Michel Fons, Estelle Devillard, Patrice Polard, Marc Maresca, Josette Perrier, Mohamed Atta, Francoise Guerlesquin, Mickaël Lafond, Victor Duarte, Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, 19168-19177. <hal-02920462>
✓
Ruminococcin C, a promising antibiotic produced by a human gut symbiont
Steve Chiumento, Clarisse Roblin, Sylvie Kieffer-Jaquinod, Sybille Tachon, Chloé Leprêtre, Christian Basset, Dwi Aditiyarini, Hamza Olleik, Cendrine Nicoletti, Olivier Bornet, Olga Iranzo, Marc Maresca, Renaud Hardré, Michel Fons, Thierry Giardina, Estelle Devillard, Françoise Guerlesquin, Yohann Couté, Mohamed Atta, Josette Perrier, Mickael Lafond, Victor Duarte, Science Advances, 2019, 5, eaaw9969. <hal-02371747>
✓
Publications
Roblin C., Chiumento S., Jacqueline C., Pinloche E., Nicoletti C., Olleik H., Courvoisier-Dezord E., Amouric A., Basset C., Dru L., Ollivier M., Bogey-Lambert A., Vidal N., Atta M., Maresca M., Devillard E., Duarte V., Perrier J., Lafond M. 2021. The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health. Int. J. Mol. Sci., 22, 3253.
https://doi.org/10.3390/ijms22063253
Roblin C.*, Chiumento S.*, Bornet O., Nouailler M., Müller C., Jeannot K., Basset C., Kieffer-Jaquinod S., Couté Y., Torelli S., Le Pape L., Schünemann V., De La Villeon B., Sockeel P., Di Pasquale E., Nicoletti C., Iranzo O., Giardina T., Fons M., Devillard E., Maresca M., Perrier J., Atta M., Guerlesquin F., Lafond M., Duarte V., 2020. The unusual structure of Ruminococcin C1 antimicrobial peptide promotes its potential therapeutic use. P.NA.S., 117 (32) 19168-19177.
https://doi.org/10.1073/pnas.2004045117
Chiumento S.*, Roblin C.*, Kieffer-Jaquinod S.*, Tachon S., Leprètre C., Basset C., Aditiyarini D., Olleik H., Nicoletti C., Bornet O., Iranzo O., Maresca M., Hardré R., Fons M., Giardina T., Devillard E., Guerlesquin F., Couté Y., Atta M., Perrier J., Lafond M., Duarte V., 2019. Ruminococcin C, a promising antibiotic produced by a human gut symbiont. Science Advances 5, eaaw9969.
https://doi.org/10.1126/sciadv.aaw9969
* These authors contributed equally to the publications.
Research Experience
Institut des Sciences Moléculaires de Marseille, France (Jan 2017 – June 2020)
Subject: Purification and characterization of the Ruminococcins C, a new family of sactipeptides and an alternative to conventionnal antibiotics
Skills: Peptides purification by chromatography (HPLC and FPLC systems), microbiological practices including on pathogens and anaerobes, identification of antimicrobial mode of action, fluorescence microscopy and image analysis
Advisors: Mickael Lafond & Josette Perrier, Estelle Devillard (Adisseo France SAS)
University of California San Diego, USA (Sept. 2015 – April 2016)
Subject: Library screening for antibiotic discovery
Skills: MIC determination, Bacterial Cytological Profiling (BCP) by fluorescence microscopy and image analysis
Advisors: Rob Knight and Joseph Pogliano
University of California San Diego, USA (Jan. – June 2015)
Subject: Development of an inducible protein degradation system in E. coli to identify novel targets for antibiotics
Skills: DNA purification, PCR, transfection and cloning, fluorescence microscopy and image analysis
Advisor: Joseph Pogliano
University of California San Diego, USA (April – Aug. 2014)
Subject: Characterization of newly isolated caves microorganisms and antibiotics extraction
Skills: 16S rRNA sequencing, chemical extraction and purification by chromatography, fluorescence microscopy
Advisor: Joseph Pogliano
McGill University, Montreal, Canada (May – Aug. 2013)
Subject: Implication of p120 RasGAP in netrin-1 mediated axon guidance
Skills: Cell culturing techniques, DNA purification, transfection, protein purification and Western-blot
Advisor: Nathalie Lamarche-Vane
Award: Merit-based CIHR (Canadian Institute of Health Research) scholarship
Laboratory of Microbiology Signals and Microenvironment (LMSM), Evreux (May – July. 2012)
Subject: Implication of TspO protein in the tolerance to several environmental stresses of P. fluorescens
Skills: DNA and RNA purification, PCR, RT-PCR
Advisor: Emeline Bouffartigues
Education
Aix-Marseille Université (2017 – 2020)
Ph.D. in Microbiology (Thesis defended on September 14th 2020)
Université Paris Diderot - Paris 7 (2013 – 2015)
M.Sc. in Genetics (with Honors)
Coursework: Genetics, Epigenetic, Genomics, Development, Cellular imaging
Université Paris Diderot - Paris 7 (2012 – 2013)
B.Sc. in Natural Sciences (with Honors)
Coursework: Genetics, Molecular biology, Cellular biology, Evolutionary genetics
University Institute of Technology, Evreux (2011 – 2012)
University Degree in Technology in Biological and Biochemical Analysis
Coursework: Molecular biology, Proteomics, Microbiology, Immunology, Toxicology, Pharmacology
Compiegne Technological University (2009 – 2011)
Engineering school
Coursework: Mathematics, Physics, Chemistry, Thermodynamics, Biology
Lycée Jeanne d’Arc, Rouen (2009)
High school diploma (with High honors)
Major in Physics and Chemistry
PhD Thesis Prize - French Society of Microbiology (SFM) - 2021
Best Poster Contribution - 6Th International Symposium AntiMicrobial Peptides - 2018
Most Creative Idea - Young Researchers Contest - Pepper School - 2018
Merit-based scolarship - Canadian Institute of Health Research - 2013
Apprentis chercheurs
Ma thèse en 180 secondes : https://www.youtube.com/watch?v=8U1rWTyxX2M
| Reference | Graphical abstract | HAL |
|---|---|---|
|
Evaluation of the Efficiency of Random and Diblock Methacrylate-Based Amphiphilic Cationic Polymers against Major Bacterial Pathogens Associated with Cystic Fibrosis |
|
✓ |
|
The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health |
|
✓ |
|
The unusual structure of Ruminococcin C1 antimicrobial peptide confers clinical properties |
|
✓ |
|
Ruminococcin C, a promising antibiotic produced by a human gut symbiont |
|
✓ |